LL21NL03 (ATCC® 57787)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: JA Garnes

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Designations LL21NL03
Species Homo sapiens, human
Depositors JA Garnes
mouse/human WAV-17 hybrid cell line
Derivation and characterization of the WAV-17 cell line were described in J. Exp.
Tissue: mouse/human WAV-17 hybrid cell line
Chromosome: 21
DNA: genomic
Digest: Partial
Insert size range: 15 kb average
Genome Equivalent: 50.0
Coverage: Clones for "complete" coverage: 3870.0
Media ATCC® Medium 1592: SM buffer
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen (volume: 0.2 ml)
Purity (human clones): 82%. Contaminants (mouse): 10.5%.
Verified to have an average insert size of 15 kb based on NotI digestion of DNA purified from the library.
Chromosomes for library construction were isolated using flow sorting.
Genome equivalents (average redundancy) were calculated by correcting for purity and assuming 15 kb average inserts and 49.2 Mb per chromosome 21.
Derivation and characterization of the WAV-17 cell line were described in J. Exp. Med. 137: 317-330, 1973; Proc. Natl. Acad. Sci. USA 81: 5504-5508, 1984 and Genomics 8: 614-622, 1990.

Trask BJ, et al. Characterization of somatic cell hybrids by bivariate flow karyotyping and fluorescence in situ hybridization. Somatic Cell Mol. Genet. 17: 117-136, 1991. PubMed: 2011792

Dunn IS, Blattner FR. Charons 36 to 40: multi enzyme, high capacity, recombination deficient replacement vectors with polylinkers and polystuffers. Nucleic Acids Res. 15: 2677-2698, 1987. PubMed: 3031608

Jeffrey A Garnes, personal communication