LL01NL01 (ATCC® 57784)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: JA Garnes

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Designations LL01NL01
Species Homo sapiens, human
Depositors JA Garnes
hamster/human HHW1113 hybrid cell line
Tissue: hamster/human HHW1113 hybrid cell line
Chromosome: 1
DNA: genomic
Digest: Partial
Insert size range: 15 kb average
Genome Equivalent: 1.480000019073486
Coverage: Clones for "complete" coverage: 23800.0
Media ATCC® Medium 1592: SM buffer
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen (volume: 0.2 ml)
Purity (human clones): 61%. Contaminants (hamster): 32%.
Verified to have an average insert size of 15 kb based on NotI digestion of DNA purified from the library.
Chromosomes for library construction were isolated using flow sorting.
Genome equivalents (average redundancy) were calculated by correcting for purity and assuming 15 kb average inserts and 257 Mb per chromosome 1.
The HHW1113 cell line was obtained from NIGMS Human Genetic Mutant Cell Repository.

Trask BJ, et al. Characterization of somatic cell hybrids by bivariate flow karyotyping and fluorescence in situ hybridization. Somatic Cell Mol. Genet. 17: 117-136, 1991. PubMed: 2011792

Dunn IS, Blattner FR. Charons 36 to 40: multi enzyme, high capacity, recombination deficient replacement vectors with polylinkers and polystuffers. Nucleic Acids Res. 15: 2677-2698, 1987. PubMed: 3031608

Jeffrey A Garnes, personal communication