LA04NS01 (ATCC® 57719)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: LL Deaven

Permits and Restrictions

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Designations LA04NS01
Species Homo sapiens, human
Depositors LL Deaven
hamster (CHO)/human lymphocyte UV20HL21-27 hybrid
This is preserved in a solution containing 10% glycerol and 2.8 M CsCl.
Tissue: hamster (CHO)/human lymphocyte UV20HL21-27 hybrid
Chromosome: 4
DNA: genomic
Digest: Complete
Insert size range: 4.0 kb average
Genome Equivalent: 1.600000023841858
Coverage: Clones for "complete" coverage: 31000.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen (volume: 0.2 ml)
This is preserved in a solution containing 10% glycerol and 2.8 M CsCl. The CsCl should be diluted to less than 40 mM before infection.
About 3% hamster impurity by in situ hybridization of sorted chromosomes; about 11% hamster impurity by plaque hybridization.
About 8% hamster impurity.
The library LA04NS01, ATCC 57719, 1.6 genomic equivalents, is distributed together with LA04NSO2, ATCC 57718, 2.3 genomic equivalents, because there is a possibility that a sequence in one may not be found in the other.
The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 2.3.

Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712