LL20NS01 (ATCC® 57712)

Clone Type: Library  /  Depositors: M Van Dilla, JA Garnes

Permits and Restrictions

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Designations LL20NS01
Depositors M Van Dilla, JA Garnes
skin fibroblast 811 cell line
Tissue: skin fibroblast 811 cell line
Chromosome: 20
DNA: genomic
Digest: Complete
Insert size range: 3.1 kb average
Genome Equivalent: 20.0
Coverage: Clones for "complete" coverage: 11000.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen (volume: 0.2 ml)
The #20 peak was well-isolated.
One of 6 unique sequence inserts did not come from chromosomes 8, 18, 19, or 20.
Of 20 probes selected for being repeat negative, 12 had inserts with repeats, 2 had inserts <500 bp, and 5/6 of the remaining unique sequence inserts hybridized to fragments of corresponding size in chromosome 20.
The value listed under genome equivalents is for the amplified library. Coverage was calculated by dividing the number of independent recombinants by the genome equivalents of the unamplified library. This value is 354.

. Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986.

Fuscoe JC, et al. Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes. Cytogenet. Cell Genet. 43: 79-86, 1986. PubMed: 3780319

Perlman J, Fuscoe JC. Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenet. Cell Genet. 43: 87-96, 1986. PubMed: 3780320

Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712

Marvin Van Dilla, personal communication