LL14NS01 (ATCC® 57706)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: M Van Dilla, JA Garnes

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Designations LL14NS01
Species Homo sapiens, human
Depositors M Van Dilla, JA Garnes
Origin
lymphoblast GM131 cell line
apparently normal
Vector
Tissue: lymphoblast GM131 cell line
Chromosome: 14
DNA: genomic
Digest: Complete
Insert size range: 4.0 kb average
Genome Equivalent: 135.0
Coverage: Clones for "complete" coverage: 17000.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen (volume: 0.2 ml)
Comments
Excellent chromosome prep; high quality flow karyotype; partial overlap of #14 and #15 peaks. Sorting purity was greater than 90% via Gaussian fit code.
References

. Human chromosome-specific DNA libraries: Construction and availability. Bio-Technology 4: 537-552, 1986.

Deaven LL, et al. Construction of human chromosome-specific DNA libraries from flow sorted chromosomes. Cold Spring Harbor Symp. Quant. Biol. 51: 159-167, 1986. PubMed: 3472712

Fuscoe JC. Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect non-recombinants. Gene 52: 291-296, 1987. PubMed: 3609744

Marvin Van Dilla, personal communication

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