Saccharomyces cerevisiae AB320 genomic library in YEp13 Escherichia coli (ATCC® 37323)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Library  /  Depositors: SI Reed, Department of Molecular Biology, Scripps Research Institute

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Designations Saccharomyces cerevisiae AB320 genomic library in YEp13 Escherichia coli
GenBank Definition

Saccharomyces cerevisiae sec11-7 suppressor Spc1p (SPC1) gene,

GenBank Number

U26257

Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors SI Reed, Department of Molecular Biology, Scripps Research Institute
Vector Name of vector: YEp13
Intact vector size (kb): 10.667
Type of vector: plasmid
Cloning sites: BamHI PvuII XhoI
Construction: pBR322, S. cerevisiae LEU2, 2 micron ori
Host range: S. cerevisiae; E. coli
Features:
promoter: none
marker(s): tetR, →, 89-1279
replicon: pMB1, 2540-2540
marker(s): ampR, <-, 3298-4158
marker(s): LEU2, →, 5843-6937
replicon: 2 micron, →, 8988-10329
Cross references: DNA Seq. Acc.: U03498
Distribution host: Escherichia coli RR1 (ATCC® 31343™)
Distributed: Frozen
Growth temperature: 37°C
Insert Type of insert: genomic
Insert size: 5-20 kb
Type of digest: Sau3A partial
Markers: ampR, LEU2
Number of independent recombinants: 8.20x104
Nonrecombinants (%): 50.0
Number of clones for complete coverage: 1.0x104
Titer: 5.9x1010 cfu/mL
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Frozen glycerol stock of Escherichia coli containing the plasmid library
Comments
A Saccharomyces cerevisiae genomic library in plasmid YEp13
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Nasmyth KA, Reed SI. Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene. Proc. Natl. Acad. Sci. USA 77: 2119-2123, 1980. PubMed: 6246523

Demianova M, et al. Yeast proteins related to the p40/laminin receptor precursor are essential components of the 40 S ribosomal subunit. J. Biol. Chem. 271: 11383-11391, 1996. PubMed: 8626693