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Species
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lethal yellowing phytoplasma
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Vector
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Construct size (kb): 4.90
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Insert
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DNA: genomic Insert lengths(kb): 2.20 Gene product: DNA Segment
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Biosafety Level
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1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
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Comments
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Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.9; BamHI--4.6; HindIII--4.1, 0.6; PstI--4.6; SalI--4.6. DNA of the lethal yellowing agent was isolated from Manila palm (Veitchia merrillii). The extent to which the lethal yellowing DNA probes hybridize to other mollicute DNAs varies according to the hybridization conditions. Hybridizes broadly to DNA of Florida MLO's. Also detects African coconut lethal decline agents associated with lethal disease (Tanzania) and Awka disease (Nigeria). Also hybridizes to DNA of the Cocos spiroplasma, other spiroplasmas and to acholeplasma DNAs.
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References
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Harrison NA, et al. DNA probes for detection of mycoplasmalike organisms associated with lethal yellowing disease of palms in Florida. Mol. Plant Pathol. 82: 216-224, 1992.
Nigel A Harrison, personal communication
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