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LYD37 (ATCC® 77323™)

Organism: lethal yellowing phytoplasma  /  Clone Type: Clone  /  Depositors: NA Harrison

Permits and Restrictions

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Designations LYD37
Species lethal yellowing phytoplasma
Depositors NA Harrison
Vector
Construct size (kb): 4.90
Insert
DNA: genomic
Insert lengths(kb): 2.20
Gene product: DNA Segment
Insert Size (kb) 2.200
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.9; BamHI--4.6; HindIII--4.1, 0.6; PstI--4.6; SalI--4.6.
DNA of the lethal yellowing agent was isolated from Manila palm (Veitchia merrillii).
The extent to which the lethal yellowing DNA probes hybridize to other mollicute DNAs varies according to the hybridization conditions.
Hybridizes broadly to DNA of Florida MLO's.
Also detects African coconut lethal decline agents associated with lethal disease (Tanzania) and Awka disease (Nigeria).
Also hybridizes to DNA of the Cocos spiroplasma, other spiroplasmas and to acholeplasma DNAs.
References

Harrison NA, et al. DNA probes for detection of mycoplasmalike organisms associated with lethal yellowing disease of palms in Florida. Mol. Plant Pathol. 82: 216-224, 1992.

Nigel A Harrison, personal communication