HTLV-II, deltaH6H11, in pBR322, purified plasmid DNA (ATCC® 45134D)

Organism: human T-cell leukemia virus II  /  Clone Type: Clone  /  Depositors: Irvin S. Y. Chen, Department of Medicine, University of California School of Medicine, Los Angeles, CA

Permits and Restrictions
Designations HTLV-II, deltaH6H11, in pBR322, purified plasmid DNA
GenBank Number

M10060

Species human T-cell leukemia virus II
Depositors Irvin S. Y. Chen, Department of Medicine, University of California School of Medicine, Los Angeles, CA
Applications
The full-length plasmid is unstable in bacterial hosts, and is distributed as purified plasmid DNA.
Vector Vector name: pBR322
Intact vector size (in kb): 4.363
Type of vector: plasmid
Vector ends: HindIII

Features :
marker(s):
tetR, ampR
replicon: pMB1
DNA Seq. Acc.: J01749
Insert Genome: human T-cell leukemia virus II
Contains complete coding sequence?: Y
Type of DNA: genomic
Insert ends: HindIII
Insert size (kb): 10.1
DNA Seq. Acc.: M10060
Biosafety Level 2
Shipping Information 200 ng dried plasmid DNA
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.2, 4.0 (doublet), 0.92, 0.36; HindIII--10.5, 3.2; XbaI--13.5.
The insert contains the following restriction sites (approximate kb from 5' end): EcoRI--0.1, 0.8, 4.7, 9.0; KpnI--1.7, 3.4; NheI--1.0, 6.65; SacI--0.5, 4.0, 8.7; XbaI--3.6; BamHI--0.3, 5.0, 8.4.
The viral portion of the insert is 8.8kb.
The viral portion of the insert is 8.8kb.
The full-length plasmid is unstable in bacterial hosts, and is distributed as purified plasmid DNA.
The viral portion of the insert is 8.8 kb. The insert also includes cellular sequences derived from Mo (T cell leukemia) cells.
Constructed by inserting the HindIII fragment from lambdaH6 into the HindIII site of pBR322 that had been modified by an EcoRV-MscI deletion of approximately 1.25 kb.
References

Chen IS, et al. Molecular characterization of genome of a novel human T-cell leukaemia virus. Nature 305: 502-505, 1983. PubMed: 6312323

Irvin S Chen, personal communication

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