pHPV-2 (6-2) (ATCC® 45022)

Organism: human papilloma virus type 2a  /  Clone Type: Clone  /  Depositors: PM Howley

Permits and Restrictions

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Designations pHPV-2 (6-2)
Species human papilloma virus type 2a
Depositors PM Howley
Vector
Construct size (kb): 12.30000019073486
Insert
DNA: genomic
Insert lengths(kb): 8.0
Tissue: DNA from human common hand wart
Gene product: human papilloma virus genome
Insert Size (kb) 8.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--8.0, 4.4; BamHI--12.4.
pHPV-2 (6-2) from common wart. Entire genomic DNA linearized at EcoRI site into EcoRI site of pBR322
Under standard conditions, no homology is detectable between HPV-1a, HPV-1b, HPV-2a, and HPV-4.
Under less stringent conditions, the HPV DNAs and BPV-1 demonstrate about 30% base mismatch.
Circular HPV-2a was linearized with EcoRI before cloning.
References

Heilman CA, et al. Cloning of human papilloma virus genomic DNAs and analysis of homologous polynucleotide sequences. J. Virol. 36: 395-407, 1980. PubMed: 6253665