pHPV-1 (3-3) (ATCC® 45021)

Organism: human papilloma virus type 1a  /  Clone Type: Clone  /  Depositors: PM Howley

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Designations pHPV-1 (3-3)
Species human papilloma virus type 1a
Depositors PM Howley
Construct size (kb): 12.30000019073486
DNA: genomic
Insert lengths(kb): 8.0
Tissue: DNA from human plantar wart
Gene product: human papilloma virus genome
Insert Size (kb) 8.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
pHPV-1 (3-3) isolated by P. Howley from a plantar wart. Entire genomic DNA linearized at BamHI site into BamHI site of pBR322.
Under standard conditions, no homology is detectable between HPV-1a, HPV-1b, HPV-2a, and HPV-4.
Under less stringent conditions, the HPV DNAs and BPV-1 demonstrate about 30% base mismatch.
Circular HPV-1a was linearized with BamHI before cloning.

Heilman CA, et al. Cloning of human papilloma virus genomic DNAs and analysis of homologous polynucleotide sequences. J. Virol. 36: 395-407, 1980. PubMed: 6253665

Gissmann L, et al. Human papilloma viruses (HPV): characterization of four different isolates. Virology 76: 569-580, 1977. PubMed: 65825