pEco63 plasmid in E. coli (ATCC® 31518)

Clone Type: Clone  /  Depositors: University of California

Permits and Restrictions

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Designations pEco63 plasmid in E. coli
Depositors University of California
Blood borne research
Sexually Transmitted Disease Research
Vector Total size of construct (kb): 9.2
Marker: ampR
Vector:  pBR325
Vector type: plasmid
Vector size (kb): 5.996
Markers: ampR, tetR
Vector ends: EcoRI
Replicon: pMB1
Insert Insert size (kb): 3.2
Gene: Hepatitis B virus
DNA: Genomic
Insert ends: EcoRI
Insert Size (kb) 3.200
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Glycerol stock of E. coli containing the plasmid
Restriction digests of the clone give the following sizes (kb): EcoRI--6.1, 3.5; HindIII--10.
Full-length clone (insert size 3.2 kb). This material is cited in a U.S. and/or other patent Application and may not be used to infringe the patent claims.
Constructed by inserting a full-length genome of hepatitis B cut with EcoRI into EcoRI site of pBR325
Constructed by inserting a full-length genome of hepatitis B virus cut with EcoRI into the EcoRI site of pBR325.

Chow YH, et al. Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2. J. Virol. 71: 169-178, 1997. PubMed: 8985336

Kate H Murashige, personal communication

Bascic Documentation
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Product Sheet
Product Sheet
Product Sheet