pBShUBC9(S) (ATCC® 99600)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: C Wang

Permits and Restrictions

View Permits

Designations pBShUBC9(S)
GenBank Number


Species Homo sapiens, human
Depositors C Wang
contains sequence ubiquitin-conjugating enzyme E2I
Construct size (kb): 4.050000190734863
Insert lengths(kb): 1.100000023841858
Tissue: placenta
Gene product: ubiquitin-conjugating enzyme E2I [UBC9]
Target Gene: ubiquitin-conjugating enzyme E2I
Insert Size (kb) 1.100
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.1; PvuII--2.6, 1.0, 0.45; XbaI--3.1, 0.88.
The insert contains the following restriction sites (approximate kb from the 5' end): NotI--0.06; AvaI--0.07; StuI--0.48; PvuII--0.94; XbaI--0.98.
ATCC 99599 and ATCC 99600 are the same for the initial 15 bp upstream of the initiation codon, the CDS, and the 3' UTR. The clones differ at the 5' end of the 5' UTR. ATCC 99599 contains a 791 bp sequence while ATCC 99600 has a different 72 bp sequence.

Wang ZY, et al. Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9. J. Biol. Chem. 271: 24811-24816, 1996. PubMed: 8798754