CD70 [CD70.1] (ATCC® 95673)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Designations CD70 [CD70.1]
GenBank Number

L08096

Species Homo sapiens, human
Applications
produces protein CD70 antigen (CD27 ligand)
Vector
Construct size (kb): 5.099999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pMT2
Intact vector size: 4.200
Type of vector: plasmid
Vector end: BstXI
Vector end: BstXI
Cloning sites: EcoRI
Polylinker sites:
Construction: pBR322; Ad VAI; Ad VAII;
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
other: non-coding DHFR
promoter: adenovirus major late
replicon: pMB1, SV40
terminator: SV4O
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CD70
Other gene symbols: CD70.1; CD27LG; CD27L
Gene name: CD70 antigen (CD27 ligand)
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19 p13
Tissue: B-lymphocyte JY cell line, EBV transformed
Type of DNA: cDNA
Insert end: Modification: BstXI adapt
Insert end: Modification: BstXI adapt
Insert size (kb): 0.89
Cross references: DNA Seq. Acc.: L08096
Nucleotides 6-888 of the insert correspond to
nucleotides 19-901 of L08096.
Insert lengths(kb): 0.8899999856948853
Tissue: B lymphocyte (EBV transformed), JY cell line
Gene product: CD70 antigen (CD27 ligand) [CD70]
Target Gene: CD70 antigen (CD27 ligand)
Insert Size (kb) 0.890
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): ApaI--5.4; EcoRI--4.4, 0.96; NheI--3.8, 1.6; SmaI--4.6, 0.76; XhoI--5.4.
The insert contains the following restrictions sites (approximate kb from the 5' end): SmaI--0.05, 0.80; NheI--0.11; XhoI--0.29; ApaI--0.37.
References

Bowman MR, et al. The cloning of CD70 and its identification as the ligand for CD27. J. Immunol. 152: 1756-1761, 1994. PubMed: 8120384

Goodwin RG, et al. Molecular and biological characterization of a ligand for CD27 defines a new family of cytokines with homology to tumor necrosis factor. Cell 73: 447-456, 1993. PubMed: 8387892