BL29-41 (ATCC® 95564)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: R Sanders

Permits and Restrictions

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Designations BL29-41
GenBank Number



Species Homo sapiens, human
Depositors R Sanders
Construct size (kb): 4.949999809265137
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Genome: Homo sapiens
Gene symbol: D6S1248E
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Chromosome: 6
Tissue: BL29 cell line
Disease: Burkitt's lymphoma
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 2.05
Cross references: DNA Seq. Acc.: T25352; EST: EST041; Primer: T7
DNA Seq. Acc.: T25353; EST: EST041R; Primer: T3
Insert lengths(kb): 2.049999952316284
Tissue: BL29 cell line Burkitt's lymphoma
Disease: Burkitt's lymphoma
Gene product: DNA Segment, single copy, expressed [D6S1248E]
Insert Size (kb) 2.050
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.35, 0.8; BamHI--3.2, 1.75; HindIII--4.1, 0.85; KpnI--5.0; PstI--3.1, 1.6, 0.3.