HO-hisG-URA3-hisG-poly-HO (ATCC® 87805)

Applications: cloning vector  /  Depositors: DJ Stillman

Permits and Restrictions

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Designations HO-hisG-URA3-hisG-poly-HO
Depositors DJ Stillman
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 8.5000000000000000
Vector: HO-hisG-URA3-hisG-poly-HO (plasmid)
Promoters: Promoter HO
Construction: pUC21
Marker(s):URA3,ampR
Construct size (kb): 8.5
Features: promoter: HO
Applications
cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): NotI--5.0, 3.5; XbaI--8.5; BamHI--8.5.
This plasmid contains two fragments from HO promoter inserted in pUC21-NotI polylinker along with hisG-URA3-hisG selectable marker. It allows for targeted integration at HO locus.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
Protocol: (LB + amp)
References

Alani E, et al. A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116: 541-545, 1987. PubMed: 3305158

David J Stillman, personal communication

Shipped frozen