HO-hisG-URA3-hisG-poly-HO (ATCC® 87805)

Applications: cloning vector  /  Depositors: DJ Stillman

Designations HO-hisG-URA3-hisG-poly-HO
Permits and Restrictions

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Depositors DJ Stillman
Biosafety Level 1
Vector Information
Size (kb): 8.5000000000000000
Vector: HO-hisG-URA3-hisG-poly-HO (plasmid)
Promoters: Promoter HO
Construction: pUC21
Construct size (kb): 8.5
Features: promoter: HO
cloning vector
Restriction digests of the clone give the following sizes (kb): NotI--5.0, 3.5; XbaI--8.5; BamHI--8.5.
This plasmid contains two fragments from HO promoter inserted in pUC21-NotI polylinker along with hisG-URA3-hisG selectable marker. It allows for targeted integration at HO locus.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
Protocol: (LB + amp)

Alani E, et al. A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116: 541-545, 1987. PubMed: 3305158

David J Stillman, personal communication

Shipped frozen