pCMV-Myc-S1P (ATCC® 87718)

Organism: Cricetus cricetus, hamster  /  Clone Type: Clone  /  Depositors: J Sakai, RB Rawson

Permits and Restrictions

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Designations pCMV-Myc-S1P
GenBank Number


Species Cricetus cricetus, hamster
Depositors J Sakai, RB Rawson
in another host, produces protein sterol-regulated site-1 protease
Construct size (kb): 10.19999980926514

Insert Information:

Insert size (kb): 4.4

Genome: Hamster

Gene symbol: S1P

Gene name: sterol-regulated site-1 protease

Type of DNA: cDNA

Genbank Accession: AF078105

Insert lengths(kb): 4.400000095367432
Gene product: sterol-regulated site-1 protease [S1P]
Target Gene: sterol-regulated site-1 protease
Insert Size (kb) 4.400
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

 Frozen glycerol stock of Escherichia coli HB101 containing the plasmid.

Restriction digests of the clone give the following sizes (kb): BamHI/XbaI -- 5.8, 4.4; EcoRI--9.7, 0.5; HindIII--6.0, 2.4, 1.8.
Insert encodes an epitope-tagged version of the protein including aa 1-23, three novel amino acids (GGR), three tandem copies of the 9E10 human c-Myc epitop tag, followed by aa 24 - 1052 of the protein.

Sakai J, et al. Molecular identification of sterol-regulated luminal protease that cleaves SREBPs and controls lipid composition of animal cells. Mol. Cell 2: 505-514, 1998. PubMed: 9809072