pMR1593 (ATCC® 87710)

Applications: cloning vector  /  Depositors: F Spencer

Designations pMR1593
Permits and Restrictions

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Depositors F Spencer
Biosafety Level 1
Vector Information
Size (kb): 7.8000001907348630
Vector: pMR1593 (phage, lambda - replacement)
Construct size (kb): 7.800000190734863
Features: marker(s): ampR, URA3
replicon: ori
MCS: SalI [delta15]
restriction site: BglII, EcoRI, PstI, NcoI, SalI, HindIII
coding sequence: karldelta15
cloning vector
Supports introduction of kar1delta15 mutation into ura3 yeast strains by two step gene replacement. The kar1delta15 mutation supports transfer of YAC clone between yeast strains by Kar1-mating (REF).
To integrate pMR1593, linearize within the kar1 gene at the unique BglII site. Cells that have lost the URA3 marker through mitotic excision can be selected on 5-fluororotic acid.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Vallen EA, et al. Separate domains of KAR1 mediate distinct functions in mitosis and nuclear fusion. J. Cell Biol. 117: 1277-1287, 1992. PubMed: 1607389

Spencer F, et al. Yeast kar1 mutants provide an effective method for YAC transfer to new hosts. Genomics 22: 118-126, 1994. PubMed: 7959757

Sano Y, et al. Sequences of ten circular ssDNA components associated with the milk vetch dwarf virus genome. J. Gen. Virol. 79: 3111-3118, 1998. PubMed: 9880029

Hugerat Y, et al. A versatile method for efficient YAC transfer between any two strains. Genomics 22: 108-117, 1994. PubMed: 7959756

Green EDYeast artificial chromosomeIn: Green EDGenome analysis: a laboratory manual3in press, 1999

Spencer F, Simchen GTransfer of YAC clones to new hosts by karyogamy deficient mating.In: Spencer F, Simchen GCurrent Protocols in Human GeneticsNew York, N.Y.John Wiley and Sonsin press, 1995

Shipped freeze-dried