REP4X (ATCC® 87604)

Applications: expression vector  /  Depositors: SL Forsburg

Permits and Restrictions

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Designations REP4X
Depositors SL Forsburg
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 8.5000000000000000
Vector: REP4X (phagemid)
Promoters: Promoter for expression nmt1 (full strength)
Construction: pUC119
Construct size (kb): 8.5
Features: marker(s): ampR
marker(s): ura4+
promoter for expression: nmt1 (full strength)
replicon: ars1
replicon: pMB1, f1
MCS: XhoI...SmaI
expression vector
Restriction digests of the clone give the following sizes (kb): XhoI--8.5; HindIII--5.5, 1.9, 1.1; EcoRI--7.1, 1.4.
REP3X (ATCC 87603) and REP4X (ATCC 87604) differ in that REP3X contains LEU2 (2.2 kb) and REP4X contains ura4+ (1.8 kb).
REP3X (ATCC 87603) and REP4X (ATCC 87604) contain full strength nmt1 promoter whereas REP41X (ATCC 87605) and REP42X (ATCC 87607) with medium strength (nmt1*) and REP81X (ATCC 87606) and REP82X (ATCC 87608) with low strength (nmt1**)
due to mutation in the TATA box by Basi et al. (Gene 123:131-136, 1993).
REP4X was constructed by deleting the ATG in the polylinker of the original REP4 plasmid. An XhoI linker was cloned into the blunted BalI-SalI cut REP4 plasmid. The SalI site is preserved.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Forsburg SL. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21: 2955-2956, 1993. PubMed: 8332516

Susan L Forsburg, personal communication

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component of:ATCC 87685
Shipped frozen
Shipping Information

 Freeze dried E. coli containing the phagemid