pTM-N (ATCC® 87197)

Applications: expression vectorvector with sequences for protein export  /  Depositors: X Liu

Permits and Restrictions

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Designations pTM-N
Depositors X Liu
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 2.8499999046325680
Vector: pTM-N (plasmid)
Promoters: Promoter for expression T7 (phi10)
Construction: pET-3a,pIN-III-ompA3
Marker(s):ampR
Construct size (kb): 2.849999904632568
Features: marker(s): ampR
other: sequence for 3' mRNA secondary structure
other: sequence for 5' mRNA secondary structure
promoter for expression: T7 (phi10)
replicon: pMB1
MCS: NcoI...BamHI
ribosome-binding site: ompA Shine-Dalgarno
signal peptide coding region: ompA
Applications
expression vector
vector with sequences for protein export
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.9; EcoRI--2.9; NcoI--2.9.
Expression vector permitting overproduction and secretion of foreign proteins in an unfused state. The ompA signal sequence directs proteins to the periplasmic space.
pTM-N differs from pTO-N (GenBank accession M34008) only in the bla (ampR) gene. The PstI site in M34008 has been removed in pTM-N.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Deng TL, et al. A novel expression vector for high-level synthesis and secretion of foreign proteins in Escherichia coli: overproduction of bovine pancreatic phospholipase A2. Gene 93: 229-234, 1990. PubMed: 2227436

Shipped freeze-dried
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