cosmid51.22 (ATCC® 79998)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: JF Gusella

Permits and Restrictions
Permits and Restrictions

View Permits

Designations cosmid51.22
GenBank Number

L01962

L01963

L01964

L01965

L01966

L01967

L01968

L01969

L01970

L01971

L01972

L01973

L01974

L01975

L01976

L01977

L01978

L01979

L01980

L01981

L01982

L01983

L01962

L01963

L01964

L01965

L01966

L01967

L01968

L01969

L01970

L01971

L01972

L01973

L01974

L01975

L01976

L01977

L01978

L01979

L01980

L01981

L01982

L01983

Species Homo sapiens, human
Depositors JF Gusella
Vector
Construct size (kb): 45.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pWE15
Intact vector size: 8.200
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI
Polylinker sites: EcoRI NotI BamHI NotI EcoRI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, kanR, G418R
promoter: T3, T7
replicon: pMB1
Cross references: DNA Seq. Acc.: X65279
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SCN4A
Gene name: sodium channel, voltage-gated, type IV, alpha polypeptide
Contains complete coding sequence?: Y
Chromosome: 17; Localization: 17 q23.1-q25.3
Tissue: lymphoblast
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 37.0
Cross references: DNA Seq. Acc.: L01962
DNA Seq. Acc.: L01963
DNA Seq. Acc.: L01964
DNA Seq. Acc.: L01965
DNA Seq. Acc.: L01966
DNA Seq. Acc.: L01967
DNA Seq. Acc.: L01968
DNA Seq. Acc.: L01969
DNA Seq. Acc.: L01970
DNA Seq. Acc.: L01971
DNA Seq. Acc.: L01972
DNA Seq. Acc.: L01973
DNA Seq. Acc.: L01974
DNA Seq. Acc.: L01975
DNA Seq. Acc.: L01976
DNA Seq. Acc.: L01977
DNA Seq. Acc.: L01978
DNA Seq. Acc.: L01979
DNA Seq. Acc.: L01980
DNA Seq. Acc.: L01981
DNA Seq. Acc.: L01982
DNA Seq. Acc.: L01983
Insert lengths(kb): 37.0
Tissue: lymphoblast
Gene product: sodium channel, voltage-gated, type IV, alpha polypeptide [SCN4A]
Insert Size (kb) 37.0
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--12.5, 10.5, 5.3, 5.0, 3.8 (doublet), 3.6, 0.8; BglII--15.5 (doublet), 12.0; EcoRI--20.0, 12.0, 8.8, 2.1; HindIII--14.5, 12.0, 7.1, 3.75, 3.6, 2.1;
SstI--13.5, 9.4, 5.6, 5.3, 3.4, 1.75, 1.35, 1.05, 0.86.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Extends from exon 1 into exon 24, from the 5' untranslated region into the 3' untranslated region.
References

McClatchey AI, et al. The genomic structure of the human skeletal muscle sodium channel gene. Hum. Mol. Genet. 1: 521-527, 1992. PubMed: 1339144

Product Sheet
Product Sheet
Product Sheet