pK931 (ATCC® 79963)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations pK931
GenBank Number

U07563

Species Homo sapiens, human
Vector
Construct size (kb): 14.19999980926514
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR328
Intact vector size: 4.900
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: AatII AsuII AvaI ClaI HgiEII HindIII Tth111I BamHI EcoRI
EcoRV BalI NcoI NruI PstI PvuI PvuII SalI SphI XmaIII
Polylinker sites:
Construction: pBR327 ori, pBR325 cmL
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, cmLR, tetR
promoter: none
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08858
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ABL1
Other gene symbols: ABL
Genomic copy number: unique
Gene name: Abelson murine leukemia viral (v-abl) oncogene homolog 1
Contains complete coding sequence?: U
Chromosome: 9; Localization: 9 q34.1
Tissue: chronic myelogenous leukemia K-562 cell line
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 9.333
Cross references: DNA Seq. Acc.: U07563
Nucleotides 1-9333 of the insert correspond to
nucleotides 8655-17987 of U07563.
Insert lengths(kb): 9.333000183105469
Tissue: chronic myelogenous leukemia K-562 cell line
Gene product: Abelson murine leukemia viral (v-abl) oncogene homolog 1 [ABL]
Alleles: D1, D2, D1, D2, A1, A2, B2, C1, C2, A1, A2, B1, B2, C1, C2, B1
Insert Size (kb) 9.333
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--14.0; EcoRI--6.2 (doublet), 1.45; HindIII--5.0, 4.4 (doublet); PstI--7.2, 1.9 (doublet), 1.8, 1.05; PvuII--4.0 (doublet), 3.9, 2.0, 0.26.
The insert contains part of the intron between exon 1b and exon 1a.
References

Grosveld G, et al. The chronic myelocytic cell line K562 contains a breakpoint in bcr and produces a chimeric bcr/c-abl transcript. Mol. Cell. Biol. 6: 607-609, 1986. PubMed: 3023859

Chissoe SL, et al. Sequence and analysis of the human ABL gene, the BCR gene, and regions involved in the Philadelphia chromosomal translocation. Genomics 27: 67-82, 1995. PubMed: 7665185

Bruce A Roe, personal communication

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