pSS38 (ATCC® 79068)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: W Stetler-Stevenson

Permits and Restrictions

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Designations pSS38
GenBank Number

J05593

Species Homo sapiens, human
Depositors W Stetler-Stevenson
Vector
Construct size (kb): 3.650000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-4
Intact vector size: 2.870
Type of vector: plasmid
Vector end: EcoRI
Vector end: XbaI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI AvaI SmaI KpnI
SacI EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI AvaI SmaI KpnI
SacI EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
MCS: EcoRI...HindIII, ->
promoter for in vitro transcription: T7, <-
replicon: pMB1
marker(s): ampR, <-
promoter for in vitro transcription: SP6, ->
Cross references: DNA Seq. Acc.: X65303
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: TIMP2
Gene name: tissue inhibitor of metalloproteinase 2
Contains complete coding sequence?: Y
Chromosome: 17; Localization: 17 q25
Tissue: A2058 melanoma cell line
Type of DNA: cDNA
Insert 5' end: EcoRI
Insert 3' end: XbaI
Insert size (kb): 0.791
Cross references: DNA Seq. Acc.: J05593
Insert lengths(kb): 0.7910000085830688
Tissue: A2058 melanoma cell line
Gene product: tissue inhibitor of metalloproteinase 2 [TIMP2]
Insert Size (kb) 0.791
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.65; EcoRI/XbaI--2.85, 0.8; HindIII--3.65; PstI--2.85, 0.8; BamHI--3.65.
The insert contains the following restriction sites (bp from the 5' end): BamHI--147; BglI--627; KpnI--338, 556; NcoI--716; PstI--26; PvuII--27.
Contains the complete coding sequence for the mature protein; nt 23-736 of the insert correspond to nt 349-1062 of the published sequence.
Detects transcripts of 3.5 and 1.0 kb.
References

Stetler-Stevenson WG, et al. Tissue inhibitor of metalloproteinases-2 (TIMP-2) mRNA expression in tumor cell lines and human tumor tissues. J. Biol. Chem. 265: 13933-13938, 1990. PubMed: 2380196

William Stetler-Stevenson, personal communication

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