pSS38 (ATCC® 79068)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: W Stetler-Stevenson

Permits and Restrictions

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Designations pSS38
GenBank Number

J05593

Species Homo sapiens, human
Depositors W Stetler-Stevenson
Vector
Construct size (kb): 3.650000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-4
Intact vector size: 2.870
Type of vector: plasmid
Vector end: EcoRI
Vector end: XbaI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI AvaI SmaI KpnI
SacI EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI AvaI SmaI KpnI
SacI EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
MCS: EcoRI...HindIII, ->
promoter for in vitro transcription: T7, <-
replicon: pMB1
marker(s): ampR, <-
promoter for in vitro transcription: SP6, ->
Cross references: DNA Seq. Acc.: X65303
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: TIMP2
Gene name: tissue inhibitor of metalloproteinase 2
Contains complete coding sequence?: Y
Chromosome: 17; Localization: 17 q25
Tissue: A2058 melanoma cell line
Type of DNA: cDNA
Insert 5' end: EcoRI
Insert 3' end: XbaI
Insert size (kb): 0.791
Cross references: DNA Seq. Acc.: J05593
Insert lengths(kb): 0.7910000085830688
Tissue: A2058 melanoma cell line
Gene product: tissue inhibitor of metalloproteinase 2 [TIMP2]
Insert Size (kb) 0.791
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.65; EcoRI/XbaI--2.85, 0.8; HindIII--3.65; PstI--2.85, 0.8; BamHI--3.65.
The insert contains the following restriction sites (bp from the 5' end): BamHI--147; BglI--627; KpnI--338, 556; NcoI--716; PstI--26; PvuII--27.
Contains the complete coding sequence for the mature protein; nt 23-736 of the insert correspond to nt 349-1062 of the published sequence.
Detects transcripts of 3.5 and 1.0 kb.
References

Stetler-Stevenson WG, et al. Tissue inhibitor of metalloproteinases-2 (TIMP-2) mRNA expression in tumor cell lines and human tumor tissues. J. Biol. Chem. 265: 13933-13938, 1990. PubMed: 2380196

William Stetler-Stevenson, personal communication

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