pSLH16 [transgene 2] (ATCC® 79013)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations pSLH16 [transgene 2]
Species Homo sapiens, human
Applications
in another host, produces protein low density lipoprotein receptor (familial hypercholesterolemia) low density lipoprotein receptor
Vector
Construct size (kb): 17.29998970031738
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pTZ18R
Intact vector size: 2.900
Type of vector: phagemid
Vector end: NotI
Vector end: NotI
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI SalI AccI HincII PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI SalI AccI HincII PstI SphI
HindIII
Construction: pUC18, Escherichia coli lacZalpha, pBR322, f1
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: LDLR
Genomic copy number: unique
Gene name: low density lipoprotein receptor (familial
hypercholesterolemia)
Contains complete coding sequence?: U
Chromosome: 19; Localization: 19 p13.2
Type of DNA: genomic
Insert end: NotI
Insert end: NotI
Insert size (kb): 14.4
Cross references:
Insert lengths(kb): 14.39999961853027
Gene product: low density lipoprotein receptor (familial hypercholesterolemia)(low density lipoprotein receptor) [LDLR]
Alleles: A2, E1, E2, A1, A2, B1, B2, D1, D2, K1, K2, Q1, Q2, S2, A1, S1
Target Gene: low density lipoprotein receptor (familial hypercholesterolemia)
Insert Size (kb) 14.400
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): SalI--14.8, 2.5; EcoRI--12.8, 4.5; BglII--14.0, 3.3; SstI--17.3; NotI--14.4; 2.9.
The insert includes the following restriction sites: SalI (intron 1, 2.0 kb from the 5' NotI site); BglII (intron 3); EcoRI (cDNA segment).
Encodes the normal sequence.
The expected size of the correctly processed mRNA is 2.9 kb.
The NotI fragment consists of the following elements: the mouse metallothionein promoter I (1.7 kb); exon 1 of LDLR, containing a SstII site; intron 1 truncated by deletion of a RsaI (77 nt 3' to exon 1) / EcoRI (3.1 kb 5' to exon 2) fragment;
exons and introns 2-4; a cDNA fragment corresponding to exons 5-18; and transcription termination and polyadenylation signals from the human growth hormone receptor gene (0.6 kb).
References

Pathak RK, et al. Tissue-specific sorting of the human LDL receptor in polarized epithelia of transgenic mice. J. Cell Biol. 111: 347-359, 1990. PubMed: 2199454

Masayuki Yokode, personal communication

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