pBTGFbeta1 [bTGF-beta1 clA]
Construct size (kb): 4.400000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDV1
Intact vector size: 3.000
Type of vector: plasmid
Cloning sites: SalI ClaI HindIII EcoRI KpnI
Construction: pBR322, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
terminator: SV40 late
DESCRIPTION OF INSERT COMPONENT:
Genome: Bos taurus
Gene symbol: TGFB1
Gene name: transforming growth factor, beta 1
Contains complete coding sequence?: U
Type of DNA: cDNA
Insert 5' end: Modification: GC
Insert 3' end: Modification: AT
Insert size (kb): 1.1
Cross references: DNA Seq. Acc.: M36271
Insert lengths(kb): 1.100000023841858
Gene product: transforming growth factor, beta 1 [TGFB1]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Restriction digests of the clone give the following sizes (kb): XhoI--2.9, 1.5; KpnI--4.4; BamHI--3.1, 1.4.
Insert contains the following restriction sites (approximate kb from the 5' end): BalI--0.57; KpnI--0.72; SmaI--0.82; PvuII--0.94.
A partial cDNA clone that lacks the 5' untranslated region and the first 223 nt of the precursor coding sequence but encodes the complete mature protein.
Van Obberghen-Schilling E, et al. Complementary deoxyribonucleic acid cloning of bovine transforming growth factor-beta1. Mol. Endocrinol. 1: 693-698, 1987. PubMed: 3153459