c9402 [9402] (ATCC® 70954)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Clone  /  Depositors: MV Olson, L Riles

Permits and Restrictions
Permits and Restrictions

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Designations c9402 [9402]
GenBank Number

Z46921

Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors MV Olson, L Riles
Applications
The ATCC encourages users of this material to communicate their results to us.
Vector
Construct size (kb): 45.41999816894531
Insert
DNA: genomic
Insert lengths(kb): 38.9900016784668
Gene product: beta-fructofuranosidase(beta-fructosidase) [SUC2]
Insert Size (kb) 38.990
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products
component of:ATCC 77284
Biosafety Level 1
Comments
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 4.025, 1.853, 0.963, 0.842, 0.469, 3.197, 1.044, 0.514, 3.708, 1.865, 0.736, 4.107, 2.971, 3.166, 0.883, 2.094, 1.324, 1.614, 0.636.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Overlaps clones with ATCC number(s): 70438, 70467, 70746.
NOTE: Because of the large number of simultaneous deposits from Dr. Olson, the ATCC has not verified that the characteristics of the material supplied here are as published. The ATCC encourages users of this material to communicate their results to us.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Westphal V, et al. Multiple pathways for facuolar sorting of yeast proteinase A. J. Biol. Chem. 271: 11865-11870, 1996. PubMed: 8662642

Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151

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Product Sheet
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