pPI-MG2 (ATCC® 69718)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: NIH, M Brantly, NIH

Permits and Restrictions
Permits and Restrictions

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Designations pPI-MG2
Species Homo sapiens, human
Depositors NIH, M Brantly, NIH
Applications
in another host, produces protein alpha-1-antitrypsin (protease inhibitor)
Vector
Construct size (kb): 11.4379997253418
Insert
DNA: genomic
Insert lengths(kb): 2.700000047683716
Gene product: alpha-1-antitrypsin (protease inhibitor) [PI]
Alleles: G3, A1, A2, B1, B2, D2, G1, G2, J2, N1, N2, C1, C2, D1, J1
Target Gene: alpha-1-antitrypsin (protease inhibitor)
Insert Size (kb) 2.700
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.1, 2.75 (doublet), 0.70; EcoRV--11.5; HindIII--5.8, 4.5, 1.05.
The insert contains the following restriction sites (approximate kb from the 5' end): EcoRV--2.26.
A minigene constructed from genomic sequence beginning 50 bp upstream of the start ATG (exon 2) and extending into exon 3, ligated to a cDNA fragment encoding exons 3-5.
References

Laubach VE, et al. Characterization of a human alpha-1-antitrypsin null allele involving aberrant mRNA splicing. Hum. Mol. Genet. 2: 1001-1005, 1993. PubMed: 8364536

Product Sheet
Product Sheet
Product Sheet