pPI-MG2 (ATCC® 69718)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: NIH, M Brantly, NIH

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Designations pPI-MG2
Species Homo sapiens, human
Depositors NIH, M Brantly, NIH
Applications
in another host, produces protein alpha-1-antitrypsin (protease inhibitor)
Vector
Construct size (kb): 11.4379997253418
Insert
DNA: genomic
Insert lengths(kb): 2.700000047683716
Gene product: alpha-1-antitrypsin (protease inhibitor) [PI]
Alleles: G3, A1, A2, B1, B2, D2, G1, G2, J2, N1, N2, C1, C2, D1, J1
Target Gene: alpha-1-antitrypsin (protease inhibitor)
Insert Size (kb) 2.700
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.1, 2.75 (doublet), 0.70; EcoRV--11.5; HindIII--5.8, 4.5, 1.05.
The insert contains the following restriction sites (approximate kb from the 5' end): EcoRV--2.26.
A minigene constructed from genomic sequence beginning 50 bp upstream of the start ATG (exon 2) and extending into exon 3, ligated to a cDNA fragment encoding exons 3-5.
References

Laubach VE, et al. Characterization of a human alpha-1-antitrypsin null allele involving aberrant mRNA splicing. Hum. Mol. Genet. 2: 1001-1005, 1993. PubMed: 8364536

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