pRB6.2 (ATCC® 65845)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations pRB6.2
Species Homo sapiens, human
Construct size (kb): 8.899999618530273
DNA: genomic
Insert lengths(kb): 6.199999809265137
Tissue: HeLa S3 cell line
Gene product: prion protein (p27-30)(prion protein) [PRIP]
Alleles: A1, A1, A1, A1, A2, A2, B1, B1, B2, B2, A2, A2
Insert Size (kb) 6.200
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Restriction digests of the clone give the following sizes (kb): BamHI--6.2, 2.7; BglI--4.2, 1.9, 1.5, 1.1, 0.3; EcoRI--9.3; HindIII--5.6, 2.5, 0.9; PvuII--4.2, 2.4 (doublet).
Derived from a cosmid containing the gene. The fragment is 8 to 9 kb 5' to exon 1. The insert also contains sequences from the BamHI site of the cosmid vector pTL5.

Puckett C, et al. Genomic structure of the human prion protein gene. Am. J. Hum. Genet. 49: 320-329, 1991. PubMed: 1678248