pRB6.2 (ATCC® 65845)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations pRB6.2
Species Homo sapiens, human
Vector
Construct size (kb): 8.899999618530273
Insert
DNA: genomic
Insert lengths(kb): 6.199999809265137
Tissue: HeLa S3 cell line
Gene product: prion protein (p27-30)(prion protein) [PRIP]
Alleles: A1, A1, A1, A1, A2, A2, B1, B1, B2, B2, A2, A2
Insert Size (kb) 6.200
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.2, 2.7; BglI--4.2, 1.9, 1.5, 1.1, 0.3; EcoRI--9.3; HindIII--5.6, 2.5, 0.9; PvuII--4.2, 2.4 (doublet).
Derived from a cosmid containing the gene. The fragment is 8 to 9 kb 5' to exon 1. The insert also contains sequences from the BamHI site of the cosmid vector pTL5.
References

Puckett C, et al. Genomic structure of the human prion protein gene. Am. J. Hum. Genet. 49: 320-329, 1991. PubMed: 1678248

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