p146-5 (ATCC® 65819)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Designations p146-5
Species Homo sapiens, human
Vector
Construct size (kb): 3.5
Insert
DNA: genomic
Insert lengths(kb): 0.5
Tissue: placenta St
Gene product: immunoglobulin kappa variable region [IGKV]
Alleles: Gd, Nd, Gd, Gd, Gd, Gp, Nd, Nd, Np, Np, Np, Gp, Gp, Nd, Gd, Nd, Gd, Nd, Gd, Nd, Gd, Nd, Np, Gp, Np, Gp, Np, Gp, Np, Gp
Insert Size (kb) 0.5
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0, 0.5; PvuII--2.5, 0.6, 0.4; PvuI--2.4, 1.1; PvuI/EcoRI--1.7, 1.1, 0.7; PvuII/XbaI--2.5, 0.4, 0.3, 0.2, 0.2.
Detects sequences from the Op (between O18 and A15) and Od (between O8 and O9) regions.
In the AccI/BamHI digests of genomic DNA, the AccI site is polymorphic.
Recloned from m146-5. The insert contains a PvuII site approximately 0.1 kb from the HindII end.
References

Pargent W, et al. Polymorphisms and haplotypes in the human immunoglobulin kappa locus. Eur. J. Immunol. 21: 1829-1835, 1991. PubMed: 1678350

Pargent W, et al. The human immunoglobulin kappa locus. Characterization of the duplicated O regions. Eur. J. Immunol. 21: 1821-1827, 1991. PubMed: 1907917

Hans G Zachau, personal communication