lambdaepsilon10EZZ (ATCC® 65692)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: M Lefranc, TH Rabbitts

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Designations lambdaepsilon10EZZ
Species Homo sapiens, human
Depositors M Lefranc, TH Rabbitts
Vector
Construct size (kb): 44.0
Insert
DNA: genomic
Insert lengths(kb): 14.39999961853027
Tissue: B lymphocyte (EBV transformed)
Gene product: immunoglobulin epsilon [IGHE]
Insert Size (kb) 14.400
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): XbaI--20.0, 11.0, 9.2, 3.8; SacI--20.0, 9.2, 3.8, 2.9, 2.3, 1.8, 1.2, 1.0, 0.65, 0.58, 0.52; XhoI--20.0, 14.0, 9.2;
SmaI--20.0, 5.8, 3.7, 3.5, 1.25, 0.90, 0.84, 0.76; PvuI--20.0, 12.0, 8.2, 5.8; HindIII--23.0, 9.2, 3.9, 0.90, 0.65.
The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--3.9, 4.8, 5.5; BamHI--7.2, 10.
Cloned from DNA isolated from individual II-4 of the TOU family.
A 3.9 kb HindIII fragment from the 5' end of this insert, pH3.9EZZ, has been deposited independently as ATCC 61230, 61231. The 5' HindIII site is vector-derived.
A 2.8 kb BamHI fragment contains the exons encoding the epsilon constant region.
References

Keyeux G, et al. A multigene deletion in the human IGH constant region locus involves highly homologous hot spots of recombination. Genomics 5: 431-441, 1989. PubMed: 2613231

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