6c (ATCC® 63128)

Organism: Mus musculus, mouse  /  Clone Type: Clone  /  Depositors: M Chu

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Designations 6c
GenBank Number


Species Mus musculus, mouse
Depositors M Chu
Construct size (kb): 4.5
Insert lengths(kb): 1.5
Tissue: Engelbreth-Holm-Swarm (EHS) tumor
Gene product: nidogen [Nid]
Alleles: B, S
Insert Size (kb) 1.5
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.5; BamHI--4.5; HindIII--4.5; PvuI--3.5, 1.0.
Restriction digests of the clone give the following sizes (kb): PvuII--2.8, 0.9, 0.8.
Contains a PstI site (nt 2627 of the published sequence) which is approximately 600 bp from the 5' EcoRI site.
Corresponds to sequences beginning about 1.5 kb upstream of the EcoRI site at nt 3525 of the published sequence, and encodes most of the rod-like domain and part of the C-terminal globular domain.
Detects mRNA of 6.0 and 4.0 kb (weak) in the mouse and 6.0 kb in the human.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

Mann K, et al. Amino acid sequence of mouse nidogen, a multidomain basement membrane protein with binding activity for laminin, collagen IV and cells. EMBO J. 8: 65-72, 1989. PubMed: 2496973

Mon-Li Chu, personal communication