M27beta [M27B, M27Beta] (ATCC® 61517)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: IW Craig

Permits and Restrictions

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Designations M27beta [M27B, M27Beta]
GenBank Number

J03073

Species Homo sapiens, human
Depositors IW Craig
Applications
A stringency of 0.5 X SSC, 64 C was used routinely.
Vector
Construct size (kb): 7.099999904632568
Insert
DNA: genomic
Insert lengths(kb): 4.400000095367432
Tissue: DNA from mouse/human MOG-T hybrid line (X only)
Gene product: DNA Segment, single copy [DXS255]
Insert Size (kb) 4.400
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
The insert contains 2 copies of a 2.2 kb insert containing the following restriction sites, separated by approximate kb: EcoRI- 0.4- HindIII- 0.1- BglII- 0.6- PvuII- 0.2- BglII- 0.9- EcoRI.
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 2.4, 2.4; HindIII--4.55, 2.4, 0.55; PvuII--2.4, 2.4, 1.4, 1.3; BamHI--7.5; HindIII--7.5.
The EcoRI RFLP shows significant linkage to Wiskott-Aldrich Syndrome (theta = 0.00, Z = 10.19).
The 0.9 BglII/EcoRI fragment contains a 334 bp HaeIII/Sau3A fragment within which a deletion of about 3 kb occurred during cloning.
The 334 bp HaeIII/Sau3A fragment contains 3 complete copies and a partial copy of a 26 bp repeat which includes a 10 bp perfect inverted repeat separated by 3 nt.
EcoRI gives the best separation of alleles on 0.8% agarose gels. A stringency of 0.5 X SSC, 64 C was used routinely.
References

Fraser NJ, et al. Multi-allelic RFLP for M27beta, an anonymous single copy genomic clone at Xp11.2-Xcen [HGM9 provisional no. DXS255]. Nucleic Acids Res. 15: 9616, 1987. PubMed: 2891116

Fraser NJ, et al. Isolation and characterization of a human variable copy number tandem repeat at Xcen-p11.2. Genomics 5: 144-148, 1989. PubMed: 2570025

Greer WL, et al. Linkage relationships of the Wiskott-Aldrich Syndrome to 10 loci in the pericentromeric region of the human X chromosome. Genomics 6: 568-571, 1990. PubMed: 2328995

Alitalo T, et al. Localization of the Aland Island Eye Disease locus to the pericentromeric region of the X chromosome by linkage analysis. Am. J. Hum. Genet. 48: 31-38, 1991. PubMed: 1985461

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