M27beta [M27B, M27Beta] (ATCC® 61517)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: IW Craig

Permits and Restrictions

View Permits

Designations M27beta [M27B, M27Beta]
GenBank Number

J03073

Species Homo sapiens, human
Depositors IW Craig
Applications
A stringency of 0.5 X SSC, 64 C was used routinely.
Vector
Construct size (kb): 7.099999904632568
Insert
DNA: genomic
Insert lengths(kb): 4.400000095367432
Tissue: DNA from mouse/human MOG-T hybrid line (X only)
Gene product: DNA Segment, single copy [DXS255]
Insert Size (kb) 4.400
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
The insert contains 2 copies of a 2.2 kb insert containing the following restriction sites, separated by approximate kb: EcoRI- 0.4- HindIII- 0.1- BglII- 0.6- PvuII- 0.2- BglII- 0.9- EcoRI.
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 2.4, 2.4; HindIII--4.55, 2.4, 0.55; PvuII--2.4, 2.4, 1.4, 1.3; BamHI--7.5; HindIII--7.5.
The EcoRI RFLP shows significant linkage to Wiskott-Aldrich Syndrome (theta = 0.00, Z = 10.19).
The 0.9 BglII/EcoRI fragment contains a 334 bp HaeIII/Sau3A fragment within which a deletion of about 3 kb occurred during cloning.
The 334 bp HaeIII/Sau3A fragment contains 3 complete copies and a partial copy of a 26 bp repeat which includes a 10 bp perfect inverted repeat separated by 3 nt.
EcoRI gives the best separation of alleles on 0.8% agarose gels. A stringency of 0.5 X SSC, 64 C was used routinely.
References

Fraser NJ, et al. Multi-allelic RFLP for M27beta, an anonymous single copy genomic clone at Xp11.2-Xcen [HGM9 provisional no. DXS255]. Nucleic Acids Res. 15: 9616, 1987. PubMed: 2891116

Fraser NJ, et al. Isolation and characterization of a human variable copy number tandem repeat at Xcen-p11.2. Genomics 5: 144-148, 1989. PubMed: 2570025

Greer WL, et al. Linkage relationships of the Wiskott-Aldrich Syndrome to 10 loci in the pericentromeric region of the human X chromosome. Genomics 6: 568-571, 1990. PubMed: 2328995

Alitalo T, et al. Localization of the Aland Island Eye Disease locus to the pericentromeric region of the X chromosome by linkage analysis. Am. J. Hum. Genet. 48: 31-38, 1991. PubMed: 1985461

Product Sheet
Product Sheet
Product Sheet