St14-1 (ATCC® 61372)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: J Mandel

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Designations St14-1
Species Homo sapiens, human
Depositors J Mandel
Construct size (kb): 7.400000095367432
DNA: genomic
Insert lengths(kb): 3.0
Gene product: DNA Segment, single copy [DXS52]
Alleles: B1, F1, F2, B2
Insert Size (kb) 3.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Restriction digests of the clone give the following sizes (kb): BamHI--7.4; EcoRI--4.4, 3.0; HindIII--4.4, 3.0; PstI--5.0, 0.96, 0.36, 0.24; HincII--4.0, 3.4.
To resolve some of the TaqI alleles with similar fragment sizes, the depositor suggests using a 0.9% agarose gel and allowing bromphenol blue migration of 15 cm.
The St14 sequence family appears to be dispersed over a 570 - 600 kb region of Xq28.
The TaqI B2 allele (4.1 + 1.4) is almost exclusively associated with the 3.4 kb allele from the VNTR polymorphism.
This probe also detects polymorphisms with MspI, DdeI, BglI, and Tth111I.
St14-1 detects genomic fragments of the following approximate sizes (kb): BamHI--3.6; HindIII--10.0; TaqI--3.9; EcoRI--10.0.

Oberle I, et al. The telomeric region of the human X chromosome long arm: presence of a highly polymorphic DNA marker and analysis of recombination frequency. Proc. Natl. Acad. Sci. USA 82: 2824-2828, 1985. PubMed: 2986139

Mandel JL, et al. Genetic mapping of the human X chromosome: linkage analysis of the q26-q28 region that includes the fragile X locus and isolation of expressed sequences. Cold Spring Harbor Symp. Quant. Biol. 51: 195-203, 1986. PubMed: 3472716

Oberle I, et al. Multipoint genetic mapping of the Xq26-q28 region in families with fragile X mental retardation and in normal families reveals tight linkage of markers in q26-q27. Hum. Genet. 77: 60-65, 1987. PubMed: 3502701

Vincent A, et al. The polymorphic marker DXS304 is within 5 centimorgans of the fragile X locus. Genomics 5: 797-801, 1989. PubMed: 2574147

Feil R, et al. Physical and genetic mapping of polymorphic loci in Xq28 (DXS15, DXS52, DXS134): analysis of a cosmid clone and a yeast artificial chromosome. Am. J. Hum. Genet. 46: 720-728, 1990. PubMed: 1969226

Barjon P, Schwartz C. New TaqI RFLPs at the DXS52 (St14) locus in the black population. Nucleic Acids Res. 17: 2149, 1989. PubMed: 2564665

Suthers GK, et al. Physical mapping of new DNA probes near the fragile X mutation (FRAXA) by using a panel of cell lines. Am. J. Hum. Genet. 47: 187-195, 1990. PubMed: 2378346

Jean Louis Mandel, personal communication

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