Y-223a (ATCC® 61170)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: U Muller, Institute of Human Genetics

Permits and Restrictions

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Designations Y-223a
Species Homo sapiens, human
Depositors U Muller, Institute of Human Genetics
Vector Construct size (kb): 5.20
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
Insert DNA: genomic
Insert lengths(kb): 0.75
Genome: Homo sapiens
Gene symbol: DYZ5
Genomic copy number: repetitive
Gene name: DNA Segment, repetitive
Contains complete coding sequence?: U
Chromosome: Y; Localization: Y p
Tissue: 49,XXXXY cell line
Type of DNA: genomic
Insert end: HindIII
Insert end: HindIII
Insert Size (kb) 0.75
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): BamHI--5.2; HindIII--4.4, 0.76; NotI--uncut; EcoRI--5.2; PstI--5.2.
Y-223a maps closer to the centromere than Y-280.
Y-223a hybridizes to DNA in 9/16 46,XX males and a female with a dicentric Y chromosome.

Muller U, et al. Deletion mapping of the testis determining locus with DNA probes in 46,XX males and in 46,XY and 46,X,dic(Y) females. Nucleic Acids Res. 14: 6489-6505, 1986. PubMed: 3748818

Gaunt SJ. Mapping of testis-determining locus on Yp by the molecular genetic analysis of XX males and XY females. Development 101: 51-58, 1987. PubMed: 88211484