m1-8 (ATCC® 61114)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: H Klobeck

Permits and Restrictions

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Designations m1-8
Species Homo sapiens, human
Depositors H Klobeck
Vector
Construct size (kb): 7.5
Insert
DNA: genomic
Insert lengths(kb): 0.3199999928474426
Tissue: placenta
Gene product: immunoglobulin kappa variable region [IGKV]
Alleles: Gd, Nd, Gd, Gd, Gd, Gp, Nd, Nd, Np, Np, Np, Gp, Gp, Nd, Gd, Nd, Gd, Nd, Gd, Nd, Gd, Nd, Np, Gp, Np, Gp, Np, Gp, Np, Gp
Insert Size (kb) 0.320
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried bacteria-free phage
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--7.2, 0.37; HindIII/PstI--7.4; BamHI--7.5; EcoRI--7.2, 0.37; HindIII--7.4; BamHI/HindIII--7.2, 0.35.
m1-8 was subcloned from m1-3. It includes part of intron 1 and most of exon 2.
m1-8 detects members of the Vkappa Subgroup I and it also cross-hybridizes with part of the VkappaIII genes.
References

Jaenichen HR, et al. Composite human Vkappa genes and a model of their evolution. Nucleic Acids Res. 12: 5249-5263, 1984. PubMed: 6087279