cMC5.2 (ATCC® 59923)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations cMC5.2
Species Homo sapiens, human
Applications
Codominant segregation was observed in 60 families.
Insert
DNA: genomic
Gene product: DNA Segment, single copy [D5S123]
Alleles: A2, A1
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--10.0, 6.2, 4.6, 3.5 (doublet), 2.1, 1.15, 0.95;
BglII--8.4, 7.0, 5.5, 4.9, 3.8, 1.4, 1.15; SalI--23, 8.4; SfiI--19, 8.3, 5.4; EcoRI--8.2, 4.8, 2.45, 1.8, 1.7, 1.1, 0.82, 0.7, 0.6, 0.45 + smaller.
Codominant segregation was observed in 60 families.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Enzyme(s) not detecting polymorphism: PstI, PvuII, MspI.
References

Weiffenbach B, et al. A genetic linkage map of human chromosome 5 with 60 RFLP loci. Genomics 10: 173-185, 1991. PubMed: 1675192

Raymond L White, personal communication