pJM128 plasmid in E. coli (ATCC® 59904)

Organism: Escherichia coli (Migula) Castellani and Chalmers  /  Clone Type: Clone  /  Depositors: TA Donlon

Permits and Restrictions

View Permits

Designations pJM128 plasmid in E. coli
Species Escherichia coli (Migula) Castellani and Chalmers
Depositors TA Donlon
Vector Total size of construct (kb): 6.95
Marker: ampR
Vector: pBR322
Vector type: plasmid
Vector size (kb): 4.4
Markers: ampR
Insert Insert size (kb): 2.55
Gene symbol: D8Z2
HGML probe ID: p03155
DNA: genomic DNA
Gene product: Alphoid repeat
Source: Human chromosome 8 cen
Insert Size (kb) 2.550
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Frozen glycerol stock of E. coli containing the plasmid
The insert contains RsaI (5), PstI (1), and XbaI (2) sites.
Restriction digests of the clone give the following sizes (kb): HindIII--4.3, 2.5; PstI--4.0, 2.9; XbaI--6.3, 0.66; XbaI/HindIII--4.3, 1.4, 0.66; XbaI/PstI--4.0, 2.2, 0.66.
This is a subclone of lambda285-1225 (the central 2.55 kb HindIII fragment) and contains 15 repeat monomers.
Classification Enterobacteriaceae, Escherichia

Donlon T, et al. Alpha satellite-like sequences at the centromere of chromosome #8. Am. J. Hum. Genet. 39: A196, 1986.

Choo KH, et al. A survey of the genomic distribution of alpha satellite DNA on all the human chromosomes, and derivation of a new consensus sequence. Nucleic Acids Res. 19: 1179-1182, 1991. PubMed: 2030938

Timothy A Donlon, personal communication