phpim5R1 [hpim-1, pHPimR1] (ATCC® 59169)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

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Designations phpim5R1 [hpim-1, pHPimR1]
Species Homo sapiens, human
Construct size (kb): 6.400000095367432
DNA: genomic
Insert lengths(kb): 3.599999904632568
Tissue: 380 cell line
Gene product: proviral integration, MCF [PIM]
Alleles: A1, A2, B1, B2, C1, A1, A2, B1, B2, C1, C2, C2
Insert Size (kb) 3.600
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 3.05; HindIII--3.6, 2.5, 0.9; PstI--2.45, 1.5, 1.2, 1.1, 0.54; BamHI--5.2, 1.2, 0.48; SalI--7.0.
Insert contains BamHI (2) and HindIII (2) sites.
Includes part of intron 4, exon 5, intron 5, exon 6, and 3' flanking sequences.
Cross-hybridizes to mouse Pim-1 gene under stringent conditions.
Vector PyT is a pBR322 derivative with a deletion between EcoRII sites 1058 and 2500.
Enzyme(s) not detecting polymorphism: EcoRI, HindIII.

Zoghbi HY, et al. Deletion and linkage mapping of eight markers from the proximal short arm of chromosome 6. Genomics 6: 352-357, 1990. PubMed: 1968423

Nagarajan L, et al. Localization of the human pim oncogene (PIM) to a region of chromosome 6 involved in translocations in acute leukemias. Proc. Natl. Acad. Sci. USA 83: 2556-2560, 1986. PubMed: 3458216

Meeker TC, et al. Characterization of the human PIM-1 gene: a putative proto-oncogene coding for a tissue specific member of the protein kinase family. Oncogene Res. 1: 87-101, 1987. PubMed: 3329711