pC63 (ATCC® 59030)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: M Litt

Permits and Restrictions

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Designations pC63
Species Homo sapiens, human
Depositors M Litt
Applications
Enzyme(s) not detecting polymorphism: TaqI, RsaI, HinfI, EcoRI, HindIII, PstI, PvuII, BglII, BamHI, SacI, BstEII (tested against 6 unrelated Caucasians).
Vector
Construct size (kb): 4.400000095367432
Insert
DNA: genomic
Insert lengths(kb): 1.700000047683716
Tissue: DNA from mouse/human PCTBA1.8 hybrid cell line
Gene product: DNA Segment, single copy [D17S21]
Alleles: A1, A2
Insert Size (kb) 1.700
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.7; SalI--4.4; BamHI--4.4; HindIII--4.4; PstI--4.4.
Codominant segregation of the MspI polymorphism has been demonstrated in Utah families K1329C and K1333 with a total of 15 children.
Enzyme(s) not detecting polymorphism: TaqI, RsaI, HinfI, EcoRI, HindIII, PstI, PvuII, BglII, BamHI, SacI, BstEII (tested against 6 unrelated Caucasians).
References

Kondoleon S, et al. An anonymous single-copy clone, pC63, from chromosome 17q23-qter identifies a frequent RFLP [HGM9 No. D17S21]. Nucleic Acids Res. 15: 9096, 1987. PubMed: 2891104

Vissing H, et al. Progress towards construction of a total restriction fragment map of a human chromosome. Nucleic Acids Res. 15: 1363-1374, 1987. PubMed: 3029713

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