754 [L754] (ATCC® 57286)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: MH Hofker

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Designations 754 [L754]
Species Homo sapiens, human
Depositors MH Hofker
Construct size (kb): 5.199999809265137
DNA: genomic
Insert lengths(kb): 2.200000047683716
Gene product: DNA Segment, single copy [DXS84]
Alleles: A2, A1
Insert Size (kb) 2.200
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Restriction digests of the clone give the following sizes (kb): HindIII--3.0, 2.2; BglI/PstI--4.0, 1.4; BglI/EcoRI--4.0, 1.4; PvuI/EcoRI--3.7, 1.6; PvuI/BamHI--3.0, 1.6, 0.9.
Shows linkage with X-linked retinitis pigmentosa (theta = 0.05, LOD score = 4.08).
The insert was isolated from a phage library derived from flow-sorted X chromosomes.

Musarella MA, et al. Localization of the gene for X-linked recessive type of retinitis pigmentosa (XLRP) to Xp21 by linkage analysis. Am. J. Hum. Genet. 43: 484-494, 1988. PubMed: 2902787

Hofker MH, et al. The X-chromosome shows less genetic variation at restriciton sites than the autosomes. Am. J. Hum. Genet. 39: 438-451, 1986. PubMed: 2876629

Bakker E, et al. Prenatal diagnosis and carrier detection of Duchenne muscular dystrophy with closely linked RFLPs. Lancet 1: 655-658, 1985. PubMed: 2858615

Hofker MH, et al. Development of additional RFLP probes near the locus for Duchenne muscular dystrophy by cosmid cloning of the DXS84 locus. Hum. Genet. 74: 270-274, 1986. PubMed: 2877935

Schwartz CE, et al. Allan-Herndon syndrome. II. Linkage to DNA markers in Xq21. Am. J. Hum. Genet. 47: 454-458, 1990. PubMed: 2393020

Thakker RV, et al. Linkage analysis of two cloned DNA sequences, DXS197 and DXS207, in hypophosphatemic rickets families. Genomics 8: 189-193, 1990. PubMed: 1979046

van Ommen GJ, et al. A physical map of 4 million bp around the Duchenne muscular dystrophy gene on the human X-chromosome. Cell 47: 499-504, 1986. PubMed: 2877741

Francke U, et al. Congenital adrenal hypoplasia, myopathy, and glycerol kinase deficiency: Molecular genetic evidence for deletions. Am. J. Hum. Genet. 40: 212-227, 1987. PubMed: 2883886

Hofker MH, et al. Isolation of probes detecting restriction fragment length polymorphisms from X chromosome-specific libraries: Potential use for diagnosis of Duchenne muscular dystrophy. Hum. Genet. 70: 148-156, 1985. PubMed: 2989153