45.1 DQbeta 021 (ATCC® 57082)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: EO Long

Permits and Restrictions

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Designations 45.1 DQbeta 021
Species Homo sapiens, human
Depositors EO Long
Vector
Construct size (kb): 4.099999904632568
Insert
DNA: cDNA
Insert lengths(kb): 1.289999961853027
Tissue: hemizygous 45.1 cell line (DR1, DQw1, DPw2)
Gene product: major histocompatibility complex, class II, DQ beta 1(major histocompatibility complex, class II, lymphocyte antigen DQB) [HLA-DQB]
Insert Size (kb) 1.290
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.7, 0.9, 0.41, 0.26; EcoRI--4.1; HindIII--4.1; SalI--3.8, 0.46; PstI--2.7, 1.2, 0.56; PstI/XhoI--1.8, 1.2, 0.9, 0.56.
The 5' end of the insert is within the TATA sequence. The insert defines the complete sequence of HLA-DWw1 beta. PstI cuts within the insert.
References

Tonnelle C, et al. DObeta: a new beta chain gene in HLA-D with a distinct regulation of expression. EMBO J. 4: 2839-2847, 1985. PubMed: 2998758

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