p9A7 (ATCC® 57010)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: WK Cavenee

Designations p9A7
Species Homo sapiens, human
Depositors WK Cavenee
Vector
Construct size (kb): 5.5
Insert
DNA: genomic
Insert lengths(kb): 1.0
Gene product: DNA Segment, single copy [D13S3]
Alleles: A2, A3, B1, A1, B2
Insert Size (kb) 1.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 1.2; BamHI--5.5; AccI--1.65, 2.0 (doublet).
DNA marker in region of deletion of chromosome 13 to which gene predisposing to retinoblastoma has been mapped.
References

Dryja TP, et al. Chromosome 13 homozygosity in osteosarcoma without retinoblastoma. Am. J. Hum. Genet. 38: 59-66, 1986. PubMed: 3004203

Radice P, et al. Loss of heterozygosity in human germinal tumors. Cytogenet. Cell Genet. 52: 72-76, 1989. PubMed: 2612217

Chidambaram A, et al. A new, low frequency MspI allele at the D13S3 locus. Nucleic Acids Res. 18: 1317, 1990. PubMed: 1690877

Lundberg C, et al. Loss of heterozygosity in human ductal breast tumors indicates a recessive mutation on chromosome 13. Proc. Natl. Acad. Sci. USA 84: 2372-2376, 1987. PubMed: 3031679

Leppert M, et al. A primary genetic map of chromosome 13q. Am. J. Hum. Genet. 39: 425-437, 1986. PubMed: 3464200