Escherichia coli (Migula) Castellani and Chalmers (ATCC® 47080)

Strain Designations: BW19094  /  Type Strain: no  /  Biosafety Level: 1

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Classification Enterobacteriaceae, Escherichia
Strain Designations BW19094
Application
transformation host
Type Strain no
Biosafety Level 1
Product Format freeze-dried
Preceptrol® no
Genotype delta(lacIPOZY)X74 uidA(deltaMlu1)::pir+ delta(phoA532) delta(phnC?DEFGHIJKLMNOP)33-30
Type Strain no
Comments
Host for maintenance of vectors containing the R6Kgamma origin of replication at low copy number.
Phenotypes verified: lac recA+ pir+.
Medium ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37.0°C
Genotype delta(lacIPOZY)X74 uidA(deltaMlu1)::pir+ delta(phoA532) delta(phnC?DEFGHIJKLMNOP)33-30
Name of Depositor BL Wanner
Special Collection NCRR Contract
References

Metcalf WW, et al. Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6K gamma origin plasmids at different copy numbers. Gene 138: 1-7, 1994. PubMed: 8125283

Barry L Wanner, personal communication

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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