pUC EJ 6.6 [pEJ6.6, pUCEJ6.6] (ATCC® 41028)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: RA Weinberg

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Designations pUC EJ 6.6 [pEJ6.6, pUCEJ6.6]
GenBank Number

J00277

Species Homo sapiens, human
Depositors RA Weinberg
Vector
Construct size (kb): 9.300000190734863
Insert
DNA: genomic
Insert lengths(kb): 6.599999904632568
Tissue: bladder carcinoma EJ cell line
Gene product: Harvey rat sarcoma viral (v-Ha-ras) oncogene homolog(Harvey rat sarcoma virus oncogene) [HRAS]
Alleles: D1, D2
Insert Size (kb) 6.600
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): XhoI--9.8; BamHI--6.6, 2.9; SacI--5.8, 3.0, 0.82.
Detects loss of heterozygosity in renal cell carcinoma.
Insert shows transforming activity--transforms NIH 3T3 cells; sequence same as in Fig. 1.
A 3 kb SacI fragment includes all exons plus 5' and 3' flanking regions.
This was subcloned from Charon 4A phi 631.
References

Iwahana H, et al. A new RFLP in intron 1 of the human c-Ha-ras1 gene and its close relationship with the variable tandem repeats in the region 3' to the gene. Oncogene 5: 1049-1053, 1990. PubMed: 1973828

Kasperczyk A, et al. Minisatellite allele diversification: the origin of rare alleles at the HRASI locus. Am. J. Hum. Genet. 47: 854-859, 1990. PubMed: 2220825

Egeland JA, et al. Bipolar affective disorders linked to DNA markers on chromosome 11. Nature 325: 783-787, 1987. PubMed: 2881209

Detera-Wadleigh SD, et al. Close linkage of c-Harvey-ras-1 and the insulin gene to affective disorder is ruled out in three North American pedigrees. Nature 325: 806-808, 1987. PubMed: 3547139

Capon DJ, et al. Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue. Nature 302: 33-37, 1983. PubMed: 6298635

Shih C, Weinberg RA. Isolation of a transforming sequence from a human bladder carcinoma cell line. Cell 29: 161-169, 1982. PubMed: 6286138

Land H, et al. Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes. Nature 304: 596-602, 1983. PubMed: 6308472

Tabin CJ, et al. Mechanism of activation of a human oncogene. Nature 300: 143-149, 1982. PubMed: 6290897

Zbar B, et al. Loss of alleles of loci on the short arm of chromosome 3 in renal cell carcinoma. Nature 327: 721-724, 1987. PubMed: 2885753

Reeve AE, et al. Loss of a Harvey ras allele in sporadic Wilms' tumor. Nature 309: 174-176, 1984. PubMed: 6325938

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