NEB620 (ATCC® 40796)

Organism: Thermococcus litoralis Neuner et al.  /  Clone Type: Clone  /  Depositors: New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc.

Permits and Restrictions
Permits and Restrictions

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Designations NEB620
GenBank Number

M74198

Species Thermococcus litoralis Neuner et al.
Depositors New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc.
Vector
Construct size (kb): 42.40000152587891
Insert
DNA: genomic
Insert lengths(kb): 1.600000023841858
Gene product: DNA polymerase (Vent) [pol]
Insert Size (kb) 1.600
Media ATCC® Medium 1592: SM buffer
Biosafety Level 1
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--20.0, 19.0, 1.9, 1.6; BglII--22.0, 12.0, 7.5, 2.4; HindIII--22.0, 9.6, 4.5, 4.0, 1.35, 1.2; PvuI--20.0, 12.0, 8.5, 2.7, 2.4; SacI--22.0, 20.0.
The content of the 1.9 kb EcoRI fragment is unknown.
The 1.6 kb EcoRI fragment encodes an internal fragment of the protein.
References

Comb DG, Kucera RB. Purified thermostable DNA polymerase obtainable from Thermococcus litoralis. US Patent 5,210,036 dated May 11 1993

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