pGH202 plasmid in Streptomyces lividans (ATCC® 39896)

Applications: produces protein endo-beta-N-acetylglucosaminidaseshuttle vectorvector with sequences for protein export endo-beta-N-acetylglucosaminidase  /  Depositors: OmniGene, Inc., S Guterman, OmniGene, Inc.

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Designations pGH202 plasmid in Streptomyces lividans
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Depositors OmniGene, Inc., S Guterman, OmniGene, Inc.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Distribution host: Streptomyces lividans
Vector Information
Size (kb): 11.30
Vector: pGH202 (plasmid)
Construction: pEHB1.6 pIJ702
Marker(s):ampR tsrR
Construct size (kb): 11.30
Features: marker(s): ampR
marker(s): tsrR
replicon: pIJ101
replicon: pMB1
signal peptide coding region: endo H
coding sequence: endo H
produces protein endo-beta-N-acetylglucosaminidase
shuttle vector
vector with sequences for protein export endo-beta-N-acetylglucosaminidase
Shuttle expression vector permitting secretion of fusion proteins from S. lividans hosts.
pGH202 (ATCC 39896) and pGH205 (ATCC 39895) differ in that the latter includes a promoter element for aminoglycoside phosphotransferase (aph).
S. lividans transformed with this plasmid will secrete endo H when grown in this medium: Difco yeast extract (3 g/L), Difco peptone (5 g/L), Oxoid malt extract (3 g/L), glucose (10 g/L), sucrose (340 g/L), and 5 mM MgCl2.
Media YEME-thiostrepton medium
Growth Conditions
Temperature: 30.0°C

Guterman S. Streptomyces secretion vector. US Patent 4,745,056 dated May 17 1988

Robbins PW, et al. Primary structure of the Streptomyces enzyme endo-beta-N-acetylglucosamine. J. Biol. Chem. 259: 7577-7583, 1984. PubMed: 6429133

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Shipping Information Distributed: freeze-dried