Size (kb): 8.3999996185302730
Vector: YEp363 (plasmid)
Promoters: Promoter none
Construction: pNM480, YEp351
Construct size (kb): 8.399999618530273
Features: insert detection: lacZ
marker(s): ampR, LEU2
replicon: pMB1, 2 micron
YE-type (episomal) shuttle vector
The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-2; SmaI-3; BamHI-3; SalI-3; PstI-1; HindIII-2. The EcoRI site is not unique.
Restriction digests of the clone give the following sizes (kb): HindIII--8.4; BamHI--8.4; SalI--8.4; PstI--8.4; EcoRI--4.25, 4.15.
One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37728
- 37730) with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame.
The sequence and reading frame of the multiple cloning sequence is: 5' GA ATT CCC GGG GAT CCG TCG ACC TGC AGC CAA GCT TGC GAT CCC 3'. The sequence begins with the first nucleotide of the MCS and ends with the CCC for amino acid 8 of beta-galactosidase.
Myers AM, et al. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. Gene 45: 299-310, 1986. PubMed: 3026915