pPA8 (ATCC® 37676)

Organism: Barley yellow dwarf luteovirus  /  Clone Type: Clone  /  Depositors: WA Miller

Permits and Restrictions

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Designations pPA8
GenBank Number


Species Barley yellow dwarf luteovirus
Depositors WA Miller
Construct size (kb): 4.400000095367432
Insert lengths(kb): 1.700000047683716
Gene product: coat protein
Insert Size (kb) 1.700
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

The insert includes the following restriction sites (with the PstI site adjacent to the HindIII site of the polylinker = 1): AvaI--151; BamHI--187; PvuII--620; AvaI--711; KpnI--870; EcoRI--876; AccI--1563; EcoRI--1683.
Restriction digests of the clone give the following sizes (kb): PstI--2.7, 1.7; EcoRI--2.9, 0.8, 0.7; HindIII--4.4.
The insert is oriented with the 5' side (3308) closer to the EcoRI site of the polylinker.
The insert includes nucleotides 3308 through 5011, encoding part of the coat protein and the overlapping 17K ORF, all of the 50K ORF, and part of the 6.7K ORF.
Classification Luteovirus

Miller WA, et al. Sequence and organization of barley yellow dwarf virus genomic RNA. Nucleic Acids Res. 16: 6097-6111, 1988. PubMed: 3399386

W A Miller, personal communication