pG181/T4 (ATCC® 37670)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Clone  /  Depositors: A Tzagoloff

Permits and Restrictions

View Permits

Designations pG181/T4
Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors A Tzagoloff
Vector
Construct size (kb): 17.0
Insert
DNA: genomic
Insert lengths(kb): 6.5
Gene product: tryptophanyl tRNA synthetase, mitochondrial
Insert Size (kb) 6.5
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
The insert contains the following sites separated by (approximate kb): BamHI- 2.0- EcoRI- 1.5- BglII- 0.28- EcoRI- 0.73- HindIII- 0.26- SphI- 0.18- BglII- 1.6- BamHI.
Restriction digests of the clone give the following sizes (kb): BamHI--17.5; KpnI--15.0, 2.6; XbaI--14.5, 1.6, 1.15; PstI/AvaI--9.0, 2.5, 2.25, 1.15, 1.05, 0.7.
The insert includes a continuous open reading frame of 1122 nt encoding a hydrophilic protein of 42 kDa. The mRNA is about 1.4 kb.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Myers AM, Tzagoloff A. MSW, a yeast gene coding for mitochondrial tryptophanyl-tRNA synthetase. J. Biol. Chem. 260: 15371-15377, 1985. PubMed: 2999114

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