pG181/T4 (ATCC® 37670)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Clone  /  Depositors: A Tzagoloff

Permits and Restrictions

View Permits

Designations pG181/T4
Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors A Tzagoloff
Construct size (kb): 17.0
DNA: genomic
Insert lengths(kb): 6.5
Gene product: tryptophanyl tRNA synthetase, mitochondrial
Insert Size (kb) 6.5
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

The insert contains the following sites separated by (approximate kb): BamHI- 2.0- EcoRI- 1.5- BglII- 0.28- EcoRI- 0.73- HindIII- 0.26- SphI- 0.18- BglII- 1.6- BamHI.
Restriction digests of the clone give the following sizes (kb): BamHI--17.5; KpnI--15.0, 2.6; XbaI--14.5, 1.6, 1.15; PstI/AvaI--9.0, 2.5, 2.25, 1.15, 1.05, 0.7.
The insert includes a continuous open reading frame of 1122 nt encoding a hydrophilic protein of 42 kDa. The mRNA is about 1.4 kb.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae

Myers AM, Tzagoloff A. MSW, a yeast gene coding for mitochondrial tryptophanyl-tRNA synthetase. J. Biol. Chem. 260: 15371-15377, 1985. PubMed: 2999114