pLKC482 (ATCC® 37596)

Applications: contains easily purifiable cassette(s) for constructionvector permitting construction of fusion proteinsvector permitting visual detection of recombinants  /  Depositors: AA Tiedman, A Shiau

Designations pLKC482
Permits and Restrictions

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Depositors AA Tiedman, A Shiau
Biosafety Level 1
Vector Information
Size (kb): 9.8999996185302730
Vector: pLKC482 (plasmid)
Promoters: Promoter none
Construction: A lacZY plasmid, Tn5
Construct size (kb): 9.899999618530273
Features: insert detection: lacZ
marker(s): ampR, kanR
promoter: none
replicon: pMB1
enhancer: none
contains easily purifiable cassette(s) for construction
vector permitting construction of fusion proteins
vector permitting visual detection of recombinants
The 6.3 kb lacZY-kanR cassette is released with SmaI.
Restriction digests of the clone give the following sizes (kb): EcoRI--10.0; BamHI--10.0; PstI--5.5, 4.4; HindIII--10.2; SmaI--6.3, 3.6.
One of a series of lacZY fusion vectors (ATCC 37594-37596) that allow fusions in one of three reading frames and retain flanking DNA for homologous recombination into the Escherichia coli genome.
A 1.3 kb HindIII/NruI kanR fragment (with HindIII site filled in) was inserted at the NruI site of a lacZY fusion plasmid.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Tiedeman AA, Smith JM. lacZY gene fusion cassettes with kanR resistance. Nucleic Acids Res. 16: 3587, 1988. PubMed: 3131742

Amelia A Tiedman, personal communication

Shipped freeze-dried