pSP65-f1+ (ATCC® 37359)

Applications: vector permitting RNA synthesis in vitrovector permitting production of single-stranded DNA  /  Depositors: DA Mead

Permits and Restrictions

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Designations pSP65-f1+
Depositors DA Mead
Biosafety Level 1
Vector Information
Size (kb): 3.5000000000000000
Vector: pSP65-f1+ (phagemid)
Promoters: Promoter SP6
Construction: bacteriophage f1, pSP65
Marker(s):ampR
Construct size (kb): 3.5
Features: marker(s): ampR
promoter: SP6
replicon: pMB1, f1
Applications
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
Comments
ssDNA SP6 promoter plasmid.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mead DA, et al. Single stranded DNA SP6 promoter plasmids for engineering mutant RNAs and proteins: synthesis of a 'stretched' preproparathyroid hormone. Nucleic Acids Res. 13: 1103-1118, 1985. PubMed: 3858795

Shipped freeze-dried
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